AuPt/NF prepared with the lattice induction of substrate was applied to construct the electrochemical immunosensor for PCT detection.
Bioelectrochemistry
; 160: 108758, 2024 Dec.
Article
de En
| MEDLINE
| ID: mdl-38878459
ABSTRACT
For the electrodeposition, the conductivity and lattice structure of substrate is important to the morphology and lattice of the deposited material. In this study, gold-platinum (AuPt) nanopartical was deposited on nickel foam (NF) based on the lattice induced orientation of the Ni substrate, and the obtained AuPt/NF was applied to construct electrochemical impedimetric immunosensor for procalcitonin (PCT) detection. As a new immunosensor matrix, NF with higher electrical conductance, flexibility and specific surface area, which can improve the plasticity, sensitivity and universality of the immunoelectrode. Due to the lattice matching between Au and Ni, ultrathin AuPt nanolayer with good biocompatibility and large surface area can be modified on the NF surface, which can bind more biomolecules and amplifies the change of impedance signal. Based on the synergistic effect between AuPt and NF, PCT detection based on this electrochemical impedimetric immunosensor with a wide linear range (0.2 pg mL-1 to 20 ng mL-1) and low detection limit (0.11 pg mL-1). In addition, this impedimetric immunosensor exhibits high recovery in the PCT detection of serum samples. This work provides a new thought and method for the construction of electrochemical immunosensor.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Platine
/
Techniques de biocapteur
/
Techniques électrochimiques
/
Limite de détection
/
Procalcitonine
/
Or
/
Nickel
Limites:
Humans
Langue:
En
Journal:
Bioelectrochemistry
/
Bioelectrochemistry (Amsterdam)
Sujet du journal:
BIOQUIMICA
Année:
2024
Type de document:
Article
Pays de publication:
Pays-Bas