Rational design of Near-Infrared fluorescent probe for monitoring HNO in plants.
Spectrochim Acta A Mol Biomol Spectrosc
; 321: 124672, 2024 Nov 15.
Article
de En
| MEDLINE
| ID: mdl-38905899
ABSTRACT
Nitroxyl (HNO), a reactive nitrogen species (RNS), is essential for plant growth. However, the action of HNO in plants has been difficult to understand due to the lack of highly sensitive and real-time in-situ monitoring tools. Herein, we presented a near-infrared fluorescent probe, DCI-HNO, based on dicyanoisophorone fluorophore, for real-time mapping HNO in plants. The introduction of a phosphine moiety as a specific HNO recognition unit can inhibit the intramolecular charge transfer (ICT) of probe DCI-HNO. However, in the presence of HNO, the ICT process occurred, leading to the emission at 665 nm. Probe DCI-HNO exhibited high sensitivity (97 nM), rapid response time (8 min), large Stokes shift (135 nm) for detection of HNO in plants. The novel developed probe has successfully imaged endogenous HNO produced during NO/H2S cross-talk in plant tissues. Additionally, the up-regulated in HNO levels during tobacco aging and in response to stress has been confirmed. Therefore, probe DCI-HNO has provided a reliable method for monitoring the NO/H2S cross-talk and revealing the role of HNO in plants.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Colorants fluorescents
/
Oxydes d'azote
Langue:
En
Journal:
Spectrochim Acta A Mol Biomol Spectrosc
/
Spectrochim. acta, Part A, Mol. biomol. spectrosc. (Print)
/
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (Print)
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2024
Type de document:
Article
Pays d'affiliation:
Chine
Pays de publication:
Royaume-Uni