Tepidibacillus marianensis sp. nov., a novel heterotrophic iron-reducing bacterium isolated from Mariana Trench sediment.
Int J Syst Evol Microbiol
; 74(6)2024 Jun.
Article
de En
| MEDLINE
| ID: mdl-38913036
ABSTRACT
A novel chemoheterotrophic iron-reducing micro-organism, designated as strain LSZ-M11000T, was isolated from sediment of the Marianas Trench. Phylogenetic analysis based on the 16S rRNA gene revealed that strain LSZ-M11000T belonged to genus Tepidibacillus, with 97â% identity to that of Tepidibacillus fermentans STGHT, a mesophilic bacterium isolated from the Severo-Stavropolskoye underground gas storage facility in Russia. The polar lipid profile of strain LSZ-M11000T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, as well as other unidentified phospholipids and lipids. The major fatty acids were C16ââ0 (28.4â%), C18ââ0 (15.8â%), iso-C15ââ0 (12.9â%), and anteiso-C15ââ0 (12.0â%). Strain LSZ-M11000T had no menaquinone. Genome sequencing revealed that the genome size of strain LSZ-M11000T was 2.97 Mb and the DNA G+C content was 37.9âmol%. The average nucleotide identity values between strain LSZ-M11000T and its close phylogenetic relatives, Tepidibacillus fermentans STGHT and Tepidibacillus decaturensis Z9T, were 76.4 and 72.6â%, respectively. The corresponding DNA-DNA hybridization estimates were 20.9 and 23.4â%, respectively. Cells of strain LSZ-M11000T were rod-shaped (1.0-1.5×0.3-0.5 µm). Using pyruvate as an electron donor, it was capable of reducing KMnO4, MnO2, As(V), NaNO3, NaNO2, Na2SO4, Na2S2O3, and K2Cr2O7. Based on phenotypic, genotypic, and phylogenetic evidence, strain LSZ-M11000T is proposed to be a novel strain of the genus Tepidibacillus, for which the name Tepdibacillus marianensis is proposed. The type strain is LSZ-M11000T (=CCAM 1008T=JCM 39431T).
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Phospholipides
/
Phylogenèse
/
Composition en bases nucléiques
/
ADN bactérien
/
ARN ribosomique 16S
/
Techniques de typage bactérien
/
Analyse de séquence d'ADN
/
Sédiments géologiques
/
Acides gras
/
Fer
Pays/Région comme sujet:
Asia
/
Europa
Langue:
En
Journal:
Int J Syst Evol Microbiol
/
Int. j. syst. evol. microbiol
/
International journal of systematic and evolutionary microbiology
Sujet du journal:
MICROBIOLOGIA
Année:
2024
Type de document:
Article
Pays de publication:
Royaume-Uni