Self-designed portable dual-mode fluorescence device with custom python-based analysis software for rapid detection via dual-color FRET aptasensor with IoT capabilities.
Food Chem
; 457: 140190, 2024 Nov 01.
Article
de En
| MEDLINE
| ID: mdl-38924915
ABSTRACT
An innovative aptasensor incorporating MoS2-modified bicolor quantum dots and a portable spectrometer, designed for the simultaneous detection of ochratoxin A (OTA) and aflatoxin B1 (AFB1) in corn was developed. Carbon dots and CdZnTe quantum dots were as nano-donors to label OTA and AFB1 aptamers, respectively. These labeled aptamers were subsequently attached to MoS2 receptors, enabling fluorescence resonance energy transfer (FRET). With targets, the labeled aptamers detached from the nano-donors, thereby disrupting the FRET process and resulting in fluorescence recovery. Furthermore, a portable dual-mode fluorescence detection system, complemented with customized python-based analysis software, was developed to facilitate rapid and convenient detection using this dual-color FRET aptasensor. The developed host program is connected to the spectrometer and transmits data to the cloud, enabling the device to have Internet of Things (IoT) characteristics. Connected to the cloud, this IoT-enabled device offers convenient and reliable fungal toxin detection for food safety.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Logiciel
/
Techniques de biocapteur
/
Contamination des aliments
/
Aflatoxine B1
/
Transfert d'énergie par résonance de fluorescence
/
Boîtes quantiques
/
Aptamères nucléotidiques
/
Ochratoxines
Langue:
En
Journal:
Food Chem
Année:
2024
Type de document:
Article
Pays de publication:
Royaume-Uni