Internal Standard Addition System for Online Breath Analysis.
Anal Chem
; 96(27): 10871-10876, 2024 07 09.
Article
de En
| MEDLINE
| ID: mdl-38937865
ABSTRACT
Breath analysis with secondary electrospray ionization (SESI) coupled to mass spectrometry (MS) is a sensitive method for breath metabolomics. To enable quantitative assessments using SESI-MS, a system was developed to introduce controlled amounts of gases into breath samples and carry out standard addition experiments. The system combines gas standard generation through controlled evaporation, humidification, breath dilution, and standard injection with the help of mass-flow controllers. The system can also dilute breath, which affects the signal of the detected components. This response can be used to filter out contaminating compounds in an untargeted metabolomics workflow. The system's quantitative capabilities have been shown through standard addition of pyridine and butyric acid into breath in real time. This system can improve the quality and robustness of breath data.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Pyridines
/
Tests d'analyse de l'haleine
/
Spectrométrie de masse ESI
Limites:
Humans
Langue:
En
Journal:
Anal Chem
Année:
2024
Type de document:
Article
Pays d'affiliation:
Suisse
Pays de publication:
États-Unis d'Amérique