The interactive effects of medicinal dyes with conventional antimicrobials against skin pathogens.
J Appl Microbiol
; 135(7)2024 Jul 02.
Article
de En
| MEDLINE
| ID: mdl-38955371
ABSTRACT
AIMS:
This study aimed to explore potential synergistic effects of medicinal dyes with antimicrobials against pathogens responsible for skin infections. METHODS ANDRESULTS:
Antimicrobial testing was conducted using minimum inhibitory concentrations and minimum bactericidal/fungicidal concentration assays. The fractional inhibitory index (ΣFIC) of combinations was calculated, and isobolograms were constructed on selected combinations. Toxicity studies were conducted using the brine-shrimp lethality assay. Combination (11 ratio) studies noted that 26% of dye-antibiotic combinations were synergistic against the Gram-positive strains, 15% against the Gram-negative strains, and 14% against the yeasts. The Mercurochrome Betadine® combination noted synergy at ratios against all the Staphylococcus aureus strains with ΣFIC values ranging from 0.05 to 0.48. The combination of Gentian violet with Gentamycin noted a 15-fold decrease in toxicity, and a selectivity index of 977.50 against the Escherichia coli (DSM 22314) strain. Time-kill studies were conducted on the combinations with the highest safe selectivity index (SI) value and lowest safe SI value i.e. Gentian violet with Gentamycin and Malachite green with Neomycin. Both combinations demonstrated better antimicrobial activity in comparison to the independent values and the controls.CONCLUSION:
This study highlights the potential for medicinal dye combinations as a treatment for skin infections.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Tests de sensibilité microbienne
/
Agents colorants
Langue:
En
Journal:
J Appl Microbiol
Sujet du journal:
MICROBIOLOGIA
Année:
2024
Type de document:
Article
Pays d'affiliation:
République d'Afrique du Sud