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Natural variation in yeast reveals multiple paths for acquiring higher stress resistance.
Scholes, Amanda N; Stuecker, Tara N; Hood, Stephanie E; Locke, Cader J; Stacy, Carson L; Zhang, Qingyang; Lewis, Jeffrey A.
Affiliation
  • Scholes AN; Department of Biological Sciences, University of Arkansas, Fayetteville, AR, USA.
  • Stuecker TN; Interdisciplinary Graduate Program in Cell and Molecular Biology, University of Arkansas, Fayetteville, AR, USA.
  • Hood SE; Department of Biological Sciences, University of Arkansas, Fayetteville, AR, USA.
  • Locke CJ; Department of Biological Sciences, University of Arkansas, Fayetteville, AR, USA.
  • Stacy CL; Department of Biological Sciences, University of Arkansas, Fayetteville, AR, USA.
  • Zhang Q; Department of Biological Sciences, University of Arkansas, Fayetteville, AR, USA.
  • Lewis JA; Interdisciplinary Graduate Program in Cell and Molecular Biology, University of Arkansas, Fayetteville, AR, USA.
BMC Biol ; 22(1): 149, 2024 Jul 04.
Article de En | MEDLINE | ID: mdl-38965504
ABSTRACT

BACKGROUND:

Organisms frequently experience environmental stresses that occur in predictable patterns and combinations. For wild Saccharomyces cerevisiae yeast growing in natural environments, cells may experience high osmotic stress when they first enter broken fruit, followed by high ethanol levels during fermentation, and then finally high levels of oxidative stress resulting from respiration of ethanol. Yeast have adapted to these patterns by evolving sophisticated "cross protection" mechanisms, where mild 'primary' doses of one stress can enhance tolerance to severe doses of a different 'secondary' stress. For example, in many yeast strains, mild osmotic or mild ethanol stresses cross protect against severe oxidative stress, which likely reflects an anticipatory response important for high fitness in nature.

RESULTS:

During the course of genetic mapping studies aimed at understanding the mechanisms underlying natural variation in ethanol-induced cross protection against H2O2, we found that a key H2O2 scavenging enzyme, cytosolic catalase T (Ctt1p), was absolutely essential for cross protection in a wild oak strain. This suggested the absence of other compensatory mechanisms for acquiring H2O2 resistance in that strain background under those conditions. In this study, we found surprising heterogeneity across diverse yeast strains in whether CTT1 function was fully necessary for acquired H2O2 resistance. Some strains exhibited partial dispensability of CTT1 when ethanol and/or salt were used as mild stressors, suggesting that compensatory peroxidases may play a role in acquired stress resistance in certain genetic backgrounds. We leveraged global transcriptional responses to ethanol and salt stresses in strains with different levels of CTT1 dispensability, allowing us to identify possible regulators of these alternative peroxidases and acquired stress resistance in general.

CONCLUSIONS:

Ultimately, this study highlights how superficially similar traits can have different underlying molecular foundations and provides a framework for understanding the diversity and regulation of stress defense mechanisms.
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Mots clés

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Saccharomyces cerevisiae / Peroxyde d'hydrogène Langue: En Journal: BMC Biol Sujet du journal: BIOLOGIA Année: 2024 Type de document: Article Pays d'affiliation: États-Unis d'Amérique Pays de publication: Royaume-Uni

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Saccharomyces cerevisiae / Peroxyde d'hydrogène Langue: En Journal: BMC Biol Sujet du journal: BIOLOGIA Année: 2024 Type de document: Article Pays d'affiliation: États-Unis d'Amérique Pays de publication: Royaume-Uni