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Mobile-CRISPRi protocol optimization for Vibrionaceae.
Geyman, Logan J; Tanner, Madeline P; Rosario-Meléndez, Natalia; Peters, Jason M; Mandel, Mark J; van Kessel, Julia C.
Affiliation
  • Geyman LJ; Department of Biology, Indiana University , Bloomington, Indiana, USA.
  • Tanner MP; Department of Biology, Indiana University , Bloomington, Indiana, USA.
  • Rosario-Meléndez N; Department of Medical Microbiology and Immunology, University of Wisconsin-Madison , Madison, Wisconsin, USA.
  • Peters JM; Microbiology Doctoral Training Program, University of Wisconsin-Madison , Madison, Wisconsin, USA.
  • Mandel MJ; Microbiology Doctoral Training Program, University of Wisconsin-Madison , Madison, Wisconsin, USA.
  • van Kessel JC; Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison , Madison, Wisconsin, USA.
Microbiol Resour Announc ; 13(8): e0004024, 2024 Aug 13.
Article de En | MEDLINE | ID: mdl-38967463
ABSTRACT
Mobile clustered regularly interspaced palindromic repeats interference (Mobile-CRISPRi) is an established method for bacterial gene expression knockdown. The deactivated Cas9 protein and guide RNA are isopropyl ß-D-1-thiogalactopyranoside inducible, and all components are integrated into the chromosome via Tn7 transposition. Here, we optimized methods specific for applying Mobile-CRISPRi in multiple Vibrio species.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Langue: En Journal: Microbiol Resour Announc Année: 2024 Type de document: Article Pays d'affiliation: États-Unis d'Amérique Pays de publication: États-Unis d'Amérique
Texte intégral
Ajouter à My VHL
Imprimer
XML
PubMed Links
Recherche sur Google

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Langue: En Journal: Microbiol Resour Announc Année: 2024 Type de document: Article Pays d'affiliation: États-Unis d'Amérique Pays de publication: États-Unis d'Amérique