Telescoping a Prenyltransferase and a Diterpene Synthase to Transform Unnatural FPP Derivatives to Diterpenoids.
Org Lett
; 26(28): 5888-5892, 2024 Jul 19.
Article
de En
| MEDLINE
| ID: mdl-38976793
ABSTRACT
New diterpenoids are accessible from non-natural FPP derivatives as substrates for an enzymatic elongation cyclization cascade using the geranylgeranyl pyrophosphate synthase (GGPPS) from Streptomyces cyaneofuscatus and the spata-13,17-diene synthase (SpS) from Streptomyces xinghaiensis. This approach led to four new biotransformation products including three new cyclododecane cores and a macrocyclic ether. For the first time, a 1,12-terpene cyclization was observed when shifting the central olefinic double bond toward the geminial methyl groups creating a nonconjugated 1,4-diene.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Streptomyces
/
Alkyl et aryl transferases
/
Dimethylallyltransferase
/
Diterpènes
Langue:
En
Journal:
Org Lett
/
Org. lett
/
Organic letters
Sujet du journal:
BIOQUIMICA
Année:
2024
Type de document:
Article
Pays d'affiliation:
Allemagne
Pays de publication:
États-Unis d'Amérique