Expression and Significance of PKM1 in Acute Myeloid Leukaemia.
J Coll Physicians Surg Pak
; 34(7): 811-816, 2024 Jul.
Article
de En
| MEDLINE
| ID: mdl-38978246
ABSTRACT
OBJECTIVE:
To investigate the expression level of pyruvate kinase M1 (PKM1) in patients with acute myeloid leukaemia (AML) as well as its clinical significance. STUDYDESIGN:
A case-control study. Place and Duration of the Study Department of Haematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China, from January 2013 to 2023.METHODOLOGY:
The expression levels of PKM1 and pyruvate kinase m2 (PKM2) in the bone marrow of 65 AML patients (excluding M3) and 31 healthy volunteers were determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), a method that measures fluorescence in real-time. The associations between PKM1, PKM2 expressions, clinical parameters, and the survival and prognosis of AML patients were analysed.RESULTS:
AML patients showed higher PKM1 expression compared to controls. The area under the curve (AUC) of the receiver operating characteristics (ROC) was 0.65 (p = 0.017). PKM1 expression was correlated with peripheral blood leukocyte count (r = -0.276, p = 0.026), CCAAT enhancer-binding protein alpha CEBPA mutation (r = -0.306, p = 0.014), and chemotherapy-induced response (r = -0.292, p = 0.018). Patients with high PKM1 expression had a lower remission rate (p = 0.019) and long-term survival rate (p = 0.034) than those with low PKM1 expression. Patients with AML showed a rise in PKM2 levels; however, the variation was not statistically significant (p >0.05).CONCLUSION:
PKM1 expression is upregulated in AML and patients with high PKM1 expression have a lower survival rate. KEY WORDS PKM1, Acute myeloid leukaemia, Clinical prognosis.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Hormones thyroïdiennes
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Leucémie aigüe myéloïde
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Protéines de transport
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Thyroid Hormone-Binding Proteins
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Protéines membranaires
Limites:
Adult
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Aged
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Female
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Humans
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Male
/
Middle aged
Pays/Région comme sujet:
Asia
Langue:
En
Journal:
J Coll Physicians Surg Pak
Sujet du journal:
MEDICINA
Année:
2024
Type de document:
Article
Pays d'affiliation:
Chine
Pays de publication:
Pakistan