Preparation of dual recognition adsorbents based on molecularly imprinted polymers and aptamer for highly sensitive recognition and enrichment of ochratoxin A.
J Hazard Mater
; 476: 135112, 2024 Sep 05.
Article
de En
| MEDLINE
| ID: mdl-38981234
ABSTRACT
In light of the significant risks that mycotoxins posed to public health and environmental safety, this research developed an adsorbent MIPs/Apt/AuNPs@ZIF-67 (MA-AZ) utilizing a dual-recognition approach combining molecularly imprinted polymers (MIPs) and aptamer (Apt). This innovative method enabled the effective and highly selective recognition and enrichment of ochratoxin A (OTA). ZIF-67 was utilized as a carrier with a substantial specific surface area, and gold nanoparticles (AuNPs) were loaded on its surface to fix the thiol-modified Apt on the surface of the carrier. Then, an initiator was used to initiate a polymerization reaction, and the generated MIPs coated Apt/AuNPs@ZIF-67, thereby synthesizing the MA-AZ with a "synergistic recognition" effect. The Apt significantly increased the number of recognition sites within the imprinted cavities, and MIPs played roles in identifying targets, fixing and protecting Apt. The combination of the both produced the effect of "1+1>2". The study on the adsorption performance of MA-AZ found that the adsorption capacity of MA-AZ could reach 65.1 mg/g, and the imprinted factor was 5.48. In addition, MA-AZ exhibited excellent stability, specificity, reusability and recovery rate. Thus, this study offers valuable insights for the recognition and enrichment of hazardous substances, and helps to promote the rapid development of safety detection.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Aptamères nucléotidiques
/
Nanoparticules métalliques
/
Polymères à empreintes moléculaires
/
Or
/
Ochratoxines
Langue:
En
Journal:
J Hazard Mater
Sujet du journal:
SAUDE AMBIENTAL
Année:
2024
Type de document:
Article
Pays de publication:
Pays-Bas