In vitro impact of Bisphenol A on the immune functions of primary cultured hemocytes of Pacific abalone (Haliotis discus hannai).
Mar Pollut Bull
; 206: 116770, 2024 Sep.
Article
de En
| MEDLINE
| ID: mdl-39053261
ABSTRACT
This study investigated the toxic effects of Bisphenol A (BPA) on the Pacific abalone (Haliotis discus hannai) using in vitro assays with primary cultured hemocytes. The abalone hemocytes were exposed to BPA concentrations up to 100 µM to assess cytotoxicity. Subsequently, hemocytes were exposed to sublethal BPA concentrations (LC20 = 2.3 µM and LC50 = 5.8 µM) for 48 h, and we evaluated the cellular immune responses of hemocytes via flow cytometry. Results showed no significant differences between LC20 and control groups, but LC50 exposure significantly reduced phagocytosis and oxidative capacities while increasing nitric oxide production. These findings suggest that BPA exposure negatively affects the immune system of the Pacific abalone, which makes them more susceptible to infections and other stressors in their natural environment. The study also implies that in vitro assays utilizing primary cultured abalone hemocytes may serve as effective proxies for quantifying the cytotoxic effects of chemical pollutants.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Phénols
/
Polluants chimiques de l'eau
/
Composés benzhydryliques
/
Gastropoda
/
Hémocytes
Limites:
Animals
Langue:
En
Journal:
Mar Pollut Bull
/
Mar. pollut. bull
/
Marine pollution bulletin
Année:
2024
Type de document:
Article
Pays de publication:
Royaume-Uni