Saponin â
from Shuitianqi () inhibits metastasis by negatively regulating the transforming growth factor-ß1/Smad7 network and epithelial-mesenchymal transition in the intrahepatic metastasis Bagg's Albino/c mouse model.
J Tradit Chin Med
; 44(4): 642-651, 2024 Aug.
Article
de En
| MEDLINE
| ID: mdl-39066524
ABSTRACT
OBJECTIVE:
To examine the influence of Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae) (SSPH I) on hepatocellular carcinoma (HCC) metastasis, and to elucidate the underlying mechanism.METHODS:
The intrahepatic metastasis Bagg's Albino/c (BALB/c) mouse model was established with human hepatocellular carcinomas (HepG2) cells, then treated with normal saline (once per day), cisplatin (2 mg/kg, once every 2 d), and SSPH â (25, 50, and 75 mg/kg, once per day). Then, we assessed alterations in the hepatic pathology and target protein expressions in the intrahepatic metastasis BALB/c mouse model using a series of molecular biology techniques.RESULTS:
Based on our analysis, SSPH â significantly alleviated hepatocyte necrosis and tumor cells infiltration. Moreover, SSPH â suppressed extracellular matrix (ECM) degradation and angiogenesis viaa decrease in matrix etalloproteinase-2 (MMP-2), MMP-9, CD31, CD34, and vascular endothelial growth factor (VEGF) levels. Furthermore, SSPH â repressed invasion and meta-stasis by suppressing the transforming growth factor-ß1 (TGF-ß1)/Smad7 axis and epithelial-mesenchymal transition (EMT), as evidenced by the scarce TGF-ß1, N-cadherin, and Vimentin expressions, and elevated Smad7 and E-cadherin expressions.CONCLUSION:
The SSPH â -mediated negative regulation of the TGF-ß1/Smad7 axis and EMT are critical for the inhibition of HCC invasion and metastasis.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Saponines
/
Médicaments issus de plantes chinoises
/
Protéine Smad7
/
Facteur de croissance transformant bêta-1
/
Transition épithélio-mésenchymateuse
/
Tumeurs du foie
Limites:
Animals
/
Humans
/
Male
Langue:
En
Journal:
J Tradit Chin Med
Année:
2024
Type de document:
Article
Pays d'affiliation:
Chine