Construction of an effective near-infrared fluorescence "turn-on" probe for hydrogen sulfide detection and imaging in living inflammatory cell and zebrafish models.
Spectrochim Acta A Mol Biomol Spectrosc
; 323: 124863, 2024 Dec 15.
Article
de En
| MEDLINE
| ID: mdl-39068845
ABSTRACT
Hydrogen sulfide (H2S) can act as a gaseous signaling mediator closely associated with inflammation development. In this work, we designed a fluorescence turn-on near-infrared (NIR) fluorescent probe CIT-H2S based on Intermolecular Charge Transfer (ICT) for the detection of H2S in living inflammatory cells and zebrafish. On this basis, a dicyanoisophorone fluorophore was chosen as the fluorescence signal reporting group of CIT-H2S, and an azide group was constructed as the recognition group of H2S. CIT-H2S is characterized by high selectivity and sensitivity for H2S over other interference species. The fluorescence intensity at 661 nm showed good linearity in the range of H2S concentration from 0 to 10 µM, with an excellent limit of detection (LOD) as low as 81.52 nM. Impressively, CIT-H2S has been visualized for detecting H2S in drug-induced inflammatory cell and zebrafish models, thus indicating that CIT-H2S is a robust tool with the ability to study the occurrence and development of hydrogen sulfide and inflammation.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Danio zébré
/
Colorants fluorescents
/
Sulfure d'hydrogène
/
Inflammation
Limites:
Animals
/
Humans
Langue:
En
Journal:
Spectrochim Acta A Mol Biomol Spectrosc
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2024
Type de document:
Article
Pays d'affiliation:
Chine
Pays de publication:
Royaume-Uni