Ca2+ as an essential signaling molecule controlling Snf1-mediated Atg1 activation.
Autophagy
; : 1-2, 2024 Aug 11.
Article
de En
| MEDLINE
| ID: mdl-39104123
ABSTRACT
Macroautophagy/autophagy is essential for maintaining glucose homeostasis, but the mechanisms by which cells sense glucose starvation and initiate autophagy are not yet fully understood. Recently, we reported that the assembly of a Ca2+-triggered Snf1-Bmh1/Bmh2-Atg11 complex initiates autophagy in response to glucose starvation. Our research reveals that during glucose starvation, the efflux of vacuolar Ca2+ increases cytoplasmic Ca2+ levels, which activates the protein kinase Rck2. Rck2-mediated phosphorylation of Atg11 enhances its interaction with Bmh1 and Bmh2. This interaction recruits the Snf1-Sip1-Snf4 complex, which is located on the vacuolar membrane, to the phagophore assembly site (PAS), leading to the activation of Atg1 and the initiation of autophagy. In summary, we have identified a previously unrecognized signaling pathway involved in glucose starvation-induced autophagy, where Ca2+ acts as a fundamental signaling molecule that links energy stress to the formation of the autophagy initiation complex.Abbreviation AMPK AMP-activated protein kinase; ATG autophagy related; co-IP co-immunoprecipitation; MAPK mitogen-activated protein kinase; PAS phagophore assembly site; ULK1 unc-51 like autophagy activating kinase 1.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Langue:
En
Journal:
Autophagy
Année:
2024
Type de document:
Article
Pays d'affiliation:
Chine