High-Pressure Freezing and Low-Temperature Processing of Seeds for Electron Microscopy and Electron Tomography.
Methods Mol Biol
; 2841: 207-214, 2024.
Article
de En
| MEDLINE
| ID: mdl-39115780
ABSTRACT
High-pressure freezing/freeze substitution has been used to preserve biological samples for ultrastructure study instead of chemical fixation. For most plant samples, the water content is too high and cannot be properly preserved during cryofixation. Additionally, the cell wall is a barrier that prevents the substitution of water with the resin. In this chapter, we will discuss modified high-pressure freezing and subsequent processing protocols based on our routinely used methodology for examining Arabidopsis seeds in transmission electron microscopy and electron tomography.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Graines
/
Arabidopsis
/
Tomographie en microscopie électronique
Langue:
En
Journal:
Methods Mol Biol
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2024
Type de document:
Article
Pays d'affiliation:
Chine