A human fetal cerebellar map of the late second trimester reveals developmental molecular characteristics and abnormality in trisomy 21.
Cell Rep
; 43(8): 114586, 2024 Aug 27.
Article
de En
| MEDLINE
| ID: mdl-39137113
ABSTRACT
Our understanding of human fetal cerebellum development during the late second trimester, a critical period for the generation of astrocytes, oligodendrocytes, and unipolar brush cells (UBCs), remains limited. Here, we performed single-cell RNA sequencing (scRNA-seq) in human fetal cerebellum samples from gestational weeks (GWs) 18-25. We find that proliferating UBC progenitors distribute in the subventricular zone of the rhombic lip (RLSVZ) near white matter (WM), forming a layer structure. We also delineate two trajectories from astrogenic radial glia (ARGs) to Bergmann glial progenitors (BGPs) and recognize oligodendrogenic radial glia (ORGs) as one source of primitive oligodendrocyte progenitor cells (PriOPCs). Additionally, our scRNA-seq analysis of the trisomy 21 fetal cerebellum at this stage reveals abnormal upregulated genes in pathways such as the cell adhesion pathway and focal adhesion pathway, which potentially promote neuronal differentiation. Overall, our research provides valuable insights into normal and abnormal development of the human fetal cerebellum.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Deuxième trimestre de grossesse
/
Cervelet
/
Syndrome de Down
/
Foetus
Limites:
Female
/
Humans
/
Pregnancy
Langue:
En
Journal:
Cell Rep
Année:
2024
Type de document:
Article
Pays de publication:
États-Unis d'Amérique