In vitrothree-dimensional volumetric printing of vitreous body models using decellularized extracellular matrix bioink.
Biofabrication
; 16(4)2024 Aug 23.
Article
de En
| MEDLINE
| ID: mdl-39142325
ABSTRACT
Hyalocytes, which are considered to originate from the monocyte/macrophage lineage, play active roles in vitreous collagen and hyaluronic acid synthesis. Obtaining a hyalocyte-compatible bioink during the 3D bioprinting of eye models is challenging. In this study, we investigated the suitability of a cartilage-decellularized extracellular matrix (dECM)-based bioink for printing a vitreous body model. Given that achieving a 3D structure and environment identical to those of the vitreous body necessitates good printability and biocompatibility, we examined the mechanical and biological properties of the developed dECM-based bioink. Furthermore, we proposed a 3D bioprinting strategy for volumetric vitreous body fabrication that supports cell viability, transparency, and self-sustainability. The construction of a 3D structure composed of bioink microfibers resulted in improved transparency and hyalocyte-like macrophage activity in volumetric vitreous mimetics, mimicking real vitreous bodies. The results indicate that our 3D structure could serve as a platform for drug testing in disease models and demonstrate that the proposed printing technology, utilizing a dECM-based bioink and volumetric vitreous body, has the potential to facilitate the development of advanced eye models for future studies on floater formation and visual disorders.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Corps vitré
/
Matrice extracellulaire
/
Bio-impression
/
Impression tridimensionnelle
/
Encre
Limites:
Animals
/
Humans
Langue:
En
Journal:
Biofabrication
Sujet du journal:
BIOTECNOLOGIA
Année:
2024
Type de document:
Article
Pays de publication:
Royaume-Uni