Coumarin-aurone based fluorescence probes for cysteine sensitive in-situ identification in living cells.
Colloids Surf B Biointerfaces
; 244: 114173, 2024 Dec.
Article
de En
| MEDLINE
| ID: mdl-39191111
ABSTRACT
The quantification of cysteine (Cys) levels in the organisms holds paramount significance in biological research and disease diagnosis, which can give the correlation between abnormal Cys levels and diseases. In this study, two fluorescent probes, designated as DEA-OH and DEA-AC, featuring a coumarin-aurone backbone specifically engineered for Cys detection, were meticulously designed and synthesized. The diethylamino coumarin-aurone probe DEA-OH and the acrylate-substituted probe DEA-AC demonstrated remarkable sensitivity in detecting cysteine by means of copper displacement (DEA-OH) and acrylate hydrolysis mechanisms (DEA-AC) with fluorescence detection limits of 7.25⯵M and 1.65⯵M, respectively. Moreover, the fluorescence peak wavelength of the two probes displayed a linear relationship with solvent polarity in the ET (30) range of 30-65â¯kcalâ¢mol-1, indicating the potential for monitoring changes in environmental polarity within this ET (30) range. The outstanding attributes exhibited by DEA-AC including superior photostability, remarkable selectivity, and swift response (kinetic rate constant 0.00747â¯s-1), coupled with the exceptional anti-interference ability, have significantly broadened its scope of applications, for example detecting alterations in Cys within biological systems.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Coumarines
/
Cystéine
/
Colorants fluorescents
Limites:
Humans
Langue:
En
Journal:
Colloids Surf B Biointerfaces
/
Colloids and surfaces
/
Colloids surf. B
Sujet du journal:
QUIMICA
Année:
2024
Type de document:
Article
Pays de publication:
Pays-Bas