Developmentally regulated mouse gene NK10 encodes a zinc finger repressor protein with differential DNA-binding domains.
DNA Cell Biol
; 14(11): 971-81, 1995 Nov.
Article
de En
| MEDLINE
| ID: mdl-7576184
ABSTRACT
Using oligonucleotides complementary to the conserved inter-finger region of a variety of previously described zinc finger-encoding genes, a novel mouse gene was cloned and characterized. The gene is localized on chromosome 8 and comprises five exons. Its corresponding mRNA is developmentally regulated in various tissues and includes an open reading frame encoding a protein of 72,422 daltons. It shares amino-terminal homologies with human KRAB (or FPB) boxes, and contains 13 zinc fingers of the C2-H2 type. The NK10 KRAB domains exhibit repressing activity when tested in GAL4 fusion protein assays. Cloning of putative target sequences revealed that the individual domains differentially contribute to zinc-dependent target DNA binding.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines de répression
/
Doigts de zinc
/
Régulation de l'expression des gènes au cours du développement
/
Protéines de liaison à l'ADN
Limites:
Animals
/
Humans
Langue:
En
Journal:
DNA Cell Biol
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
1995
Type de document:
Article
Pays d'affiliation:
Allemagne