Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes.
Gene
; 160(1): 81-6, 1995 Jul 04.
Article
de En
| MEDLINE
| ID: mdl-7628722
ABSTRACT
We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZ alpha-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the KmR gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Plasmides
/
Résistance microbienne aux médicaments
/
Clonage moléculaire
/
Actinobacillus pleuropneumoniae
/
Escherichia coli
/
Gènes bactériens
/
Vecteurs génétiques
Langue:
En
Journal:
Gene
Année:
1995
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique