Effects of bromohomoibotenate on metabotropic glutamate receptors.
Neuroreport
; 5(18): 2417-20, 1994 Dec 20.
Article
de En
| MEDLINE
| ID: mdl-7696571
ABSTRACT
(S)-Bromohomoibotenic acid [(S)-BrHIbo] stereoselectively antagonized glutamate-stimulated phosphoinositide (PI) hydrolysis in baby hamster kidney (BHK) cells expressing mGluR1a in a competitive manner with an IC50 of 250 microM. However, (S)-BrHIbo did not inhibit (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid [(1S,3R)-ACPD]-induced PI hydrolysis in rat hippocampal slices (S)- or (R)-BrHIbo did not show any effects on forskolin-stimulated cAMP-formation in BHK cells expressing mGluR2 or mGluR4 but did displace [3H]2-amino-4-phosphonobutyrate ([3H]AP4) binding from rat corticalmembranes with high affinities (IC50 = 1.0 microM and 1.1 microM, respectively). These data suggest that (S)-BrHIbo may interest with multiple PI-coupled glutamate receptors, however, at concentrations that are several fold higher than for displacement of [3H]AP4 binding from rat cortical membranes.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Récepteurs métabotropes au glutamate
/
Acide iboténique
/
Rein
Limites:
Animals
Langue:
En
Journal:
Neuroreport
Sujet du journal:
NEUROLOGIA
Année:
1994
Type de document:
Article
Pays d'affiliation:
Danemark