The beta-adrenoceptor selectivity profile of BRL 37344 in the pithed rat.
J Auton Pharmacol
; 14(5): 337-44, 1994 Oct.
Article
de En
| MEDLINE
| ID: mdl-7829538
ABSTRACT
1. Rats were pithed in order to disrupt baroreflex pathways. Heart rate was used as a measure of beta 1-adrenoceptor activity, blood pressure as a measure of beta 2-adrenoceptor activity and oxygen consumption and brown adipose tissue temperature as measures of beta 3-adrenoceptor activity. 2. The effects of the selective beta 3-adrenoceptor agonist BRL 37344 were compared with those of isoprenaline, a non-selective beta-adrenoceptor agonist, and denopamine and salbutamol, which are respectively beta 1 and beta 2-adrenoceptor agonists. 3. Denopamine was 10-fold more potent on heart rate than blood pressure, whilst salbutamol was 18-fold more potent on blood pressure than heart rate. These findings confirm that in this preparation increases in heart rate are predominantly beta 1 adrenoceptor-mediated, whilst blood pressure is beta 2-adrenoceptor-mediated. Further confirmation is provided by the blockade with atenolol, of the chronotropic effect, but not the blood pressure effect, of isoprenaline. 4. BRL 37344 was the most potent beta-adrenoceptor agonist on both oxygen consumption and brown adipose tissue temperature, revealing the beta 3-nature of these responses. Dose-response curves for oxygen consumption and brown adipose tissue temperature were identical, whichever of the beta-adrenoceptor agonists was used. Both systems may be considered equally effective as indicators of beta 3-adrenoceptor agonist activity.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Récepteurs bêta-adrénergiques
/
Agonistes bêta-adrénergiques
/
Éthanolamines
Limites:
Animals
Langue:
En
Journal:
J Auton Pharmacol
Année:
1994
Type de document:
Article