[Phagotest and Bursttest (Phagoburst), test kits for study of phagocyte functions]. / Phagotest und Bursttest (Phagoburst), Testkits zur Untersuchung von Phagozytenfunktionen.
Wien Klin Wochenschr
; 106(8): 250-2, 1994.
Article
de De
| MEDLINE
| ID: mdl-8023517
ABSTRACT
Phagotest and Bursttest are complete test kits for the investigation of the phagocytic function of granulocytes and monocytes in whole blood using flow cytometry. Phagotest allows the quantitative determination of leukocyte phagocytosis (uptake of bacteria). It determines the percentage of phagocytes which ingest fluorescein-isothiocyanate (FITC) labelled opsonized E. coli bacteria and their activity (number of bacteria per cell). Reduced phagocytosis is observed in patients with sepsis, renal failure, and recurrent bacterial infections. Bursttest allows the quantitative determination of leukocyte oxidative burst. It determines the percentage of leukocytes which oxidize the fluorogenic substrate dihydrorhodamine (DHR) 123 and their enzymatic activity (amount of rhodamine 123). Reduced burst activity is observed in patients with chronic granulomatous disease (CGD).
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Phagocytose
/
Trousses de réactifs pour diagnostic
/
Monocytes
/
Stimulation du métabolisme oxydatif
/
Cytométrie en flux
/
Granulocytes
Langue:
De
Journal:
Wien Klin Wochenschr
Année:
1994
Type de document:
Article