Interleukin-1 beta concentration of gingival crevicular fluid.
J Periodontol
; 65(5): 423-8, 1994 May.
Article
de En
| MEDLINE
| ID: mdl-8046557
ABSTRACT
The concentration of interleukin-1 beta is elevated in inflamed gingival tissue. Therefore a method for the measurement of interleukin-1 beta (Il-1 beta) in gingival crevicular fluid (GCF) using a commercially available Il-1 beta ELISA was developed. GCF was collected with periopaper strips and 4 protocols of sampling using filter paper strips were tested; the method with a recovery rate of 111.9% (SD +/- 14.5%) was chosen for subsequent analysis of all samples. Il-1 beta concentration in GCF of periodontitis patients and a healthy control group was determined. Patients (n = 19, mean age 29.3 years) had not been treated. The healthy control group (n = 14, mean age 22.8 years) showed, after a hygiene regimen of 2 weeks, no clinical signs of gingival/periodontal inflammation. Probing depth, clinical attachment level, bleeding upon probing, and a modified plaque index were recorded. Il-1 beta could be detected in all GCF samples. The concentration ranged between 22.8 ng/ml and 150 ng/ml in the healthy control group and between 85.8 ng/ml and 882.2 ng/ml in the periodontitis patients. No sex-related differences were noted. According to our present results the determination of GCF Il-1 beta concentration is possible using commercially available test kits if the principle of sample preparation is adapted to the specific requirements of GCF analysis.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Parodontite
/
Exsudat gingival
/
Interleukine-1
Type d'étude:
Guideline
Limites:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Langue:
En
Journal:
J Periodontol
Année:
1994
Type de document:
Article
Pays d'affiliation:
Allemagne