[3H]GBR-12935 binding to human cerebral cortex is not to dopamine uptake sites.
J Neurochem
; 62(1): 338-41, 1994 Jan.
Article
de En
| MEDLINE
| ID: mdl-8263533
ABSTRACT
The binding of the dopamine uptake inhibitor [3H]GBR-12935 to 16 regions of the human brain was investigated in competition experiments with increasing concentrations of GBR-12909, mazindol, and dopamine. The methodology used included a relatively high tissue concentration (8 mg/ml) and addition of 5 mM KCl in the assay buffer. GBR-12909 inhibited 80-90% of the binding in most regions, whereas dopamine only inhibited the binding in the striatum. Mazindol inhibited only part of the cortical binding at concentrations of > 1 microM, whereas the inhibition in the caudate and the putamen also contained a high-affinity component representing the dopamine uptake site. It is concluded that the [3H]GBR-12935 binding sensitive to GBR-12909 cannot be regarded as specific binding to the dopamine uptake site because the displaceable binding most likely is not related to the dopamine uptake site.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Pipérazines
/
Dopamine
/
Cortex cérébral
Limites:
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Langue:
En
Journal:
J Neurochem
Année:
1994
Type de document:
Article
Pays d'affiliation:
Suède