Three-dimensional analysis of mineralizing turkey leg tendon: matrix vesicle-collagen relationships.
Matrix Biol
; 14(7): 543-52, 1995 Jul.
Article
de En
| MEDLINE
| ID: mdl-8535604
ABSTRACT
The spatial and temporal relationships of mineral deposition between matrix vesicles and type I collagen fibrils have been studied in the turkey leg tendon by electron microscopy of cross sections and serial longitudinal thin sections and by electron tomography of longitudinal thick sections. Serial sectioning and electron tomography allow three-dimensional analysis of spatial relationships, overcoming the problems of missing depth information and over-projection of adjacent structures which exist for two-dimensional projections of isolated sections. These techniques reveal that while mineral deposits within matrix vesicles are found remote from calcifying collagen fibrils, the reverse relationship does not occur; all collagen-associated mineral can ultimately be linked to mineral-laden vesicles. These results suggest a temporal sequence of calcification beginning in matrix vesicles and spreading to adjacent collagen fibrils.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Tendons
/
Collagène
/
Matrice extracellulaire
/
Membre pelvien
/
Minéraux
Limites:
Animals
Langue:
En
Journal:
Matrix Biol
Sujet du journal:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Année:
1995
Type de document:
Article
Pays d'affiliation:
Canada