Immunogenicity and safety of recombinant Helicobacter pylori urease in a nonhuman primate.
Dig Dis Sci
; 41(9): 1853-62, 1996 Sep.
Article
de En
| MEDLINE
| ID: mdl-8794806
ABSTRACT
Groups of squirrel monkeys (Saimiri spp.), predetermined to be free of Helicobacter infections in the gastric mucosa, were immunized orally with 0.5-4.5 mg of Helicobacter pylori recombinant urease (rUrease) and 25-500 micrograms of Escherichia coli heat-labile enterotoxin (LT) adjuvant. Oral immunization with rUrease resulted in a markedly elevated serum immunoglobulin G (IgG) antibody response with peak levels at 45 days after immunization. No significant gastric inflammation or cytotoxicity was evident in rUrease immunized monkeys as determined by light and electron microscopy. Twenty-five micrograms of LT was a sufficient and safe adjuvant dosage, whereas higher dosages resulted in diarrhea and lethargy. Animals developed a serum IgG antibody response to LT that did not impede the production of anti-rUrease antibody levels. The results of this investigation indicate that rUrease is immunogenic in a nonhuman primate.
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Toxines bactériennes
/
Urease
/
Immunoglobuline G
/
Adjuvants immunologiques
/
Protéines Escherichia coli
/
Entérotoxines
Limites:
Animals
Langue:
En
Journal:
Dig Dis Sci
Année:
1996
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique