Similar effects of alpha- and beta-SNAP on Ca(2+)-regulated exocytosis.
FEBS Lett
; 393(2-3): 185-8, 1996 Sep 16.
Article
de En
| MEDLINE
| ID: mdl-8814286
ABSTRACT
Soluble N-ethylmaleimide-sensitive fusion protein attachment proteins (SNAP) proteins function in Ca(2+)-regulated exocytosis. Recent work (Schiavo et al. (1996) Nature 378, 733-736) based on in vitro protein interactions has raised the possibility that alpha- and beta-SNAPs have distinct roles in exocytosis. We have examined this possibility by comparing the activities of recombinant alpha- and beta-SNAPs. Both of these proteins were able to similarly bind NSF and activate its ATPase activity but to a lesser extent than gamma-SNAP. When introduced into digitoninpermeabilised chromaffin cells, both alpha- and beta-SNAP stimulated Ca(2+)-regulated exocytosis in a MgATP-dependent manner. The dose-response relationships for these proteins were essentially the same and addition of both proteins did not lead to any further increase in exocytosis above that due to each protein alone. We conclude that alpha- and beta-SNAPs are interchangeable isoforms with similar functions in regulated exocytosis.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines de transport
/
Calcium
/
Cellules chromaffines
/
Protéines du transport vésiculaire
/
Exocytose
/
Protéines membranaires
Limites:
Animals
Langue:
En
Journal:
FEBS Lett
Année:
1996
Type de document:
Article
Pays d'affiliation:
Royaume-Uni