Proteolysis of ankyrin and Na+/K(+)-ATPase in postmortem rat brain: is calpain involved?
Forensic Sci Int
; 86(1-2): 77-85, 1997 Apr 18.
Article
de En
| MEDLINE
| ID: mdl-9153785
ABSTRACT
Ankyrin links the fodrin-based cytoskeleton to membrane proteins such as Na+/K(+)-ATPase, thereby maintaining cellular integrity. Immunoblotting by antibody raised against erythrocyte ankyrin demonstrated the proteolysis of ankyrin, which was highly correlated with postmortem interval (0-24 h). Proteolysis in the postmortem brain generated the 160-kDa fragment with an identical size as the fragment formed after in vitro proteolysis by calpain. Although microM Ca2+ induced the proteolysis in the homogenate, the presence of mu-calpain was not demonstrated by immunoblotting using the antibody that reacts with large subunits both of mu- and m-calpains. Na+/K(+)-ATPase was also proteolyzed in the postmortem brain.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Modifications postmortem
/
Encéphale
/
Calpain
/
Ankyrines
/
Sodium-Potassium-Exchanging ATPase
Limites:
Animals
Langue:
En
Journal:
Forensic Sci Int
Année:
1997
Type de document:
Article
Pays d'affiliation:
Japon