Internalization of Aeromonas hydrophila by fish epithelial cells can be inhibited with a tyrosine kinase inhibitor.
Microbiology (Reading)
; 144 ( Pt 2): 299-307, 1998 Feb.
Article
de En
| MEDLINE
| ID: mdl-9493367
ABSTRACT
Aeromonas hydrophila is a Gram-negative bacterium that is pathogenic in fish, causing motile aeromonad septicaemia. It can enter (invade) fish cells, and survive as an intracellular parasite. The host-pathogen interaction and signal transduction pathway were studied by screening signal transduction inhibitors using carp epithelial cells and a virulent strain of the bacterium, PPD134/91. Genistein, a tyrosine kinase inhibitor, postponed internalization of A. hydrophila into host cells, suggesting that tyrosine phosphorylation plays a role in internalization. In contrast, staurosporine, a protein kinase C inhibitor, and sodium orthovanadate, a protein tyrosine phosphatase inhibitor, accelerated internalization of PPD134/91. Other virulent strains of A. hydrophila were also examined and it is likely that all strains, irrespective of serogroup, use the same signalling pathway to facilitate bacterial uptake.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protein-tyrosine kinases
/
Infections bactériennes à Gram négatif
/
Aeromonas hydrophila
/
Génistéine
/
Antienzymes
Limites:
Animals
Langue:
En
Journal:
Microbiology (Reading)
Sujet du journal:
MICROBIOLOGIA
Année:
1998
Type de document:
Article