Phylogenetic position of rare human mycoplasmas, Mycoplasma faucium, M. buccale, M. primatum and M. spermatophilum, based on 16S rRNA gene sequences.
Int J Syst Bacteriol
; 48 Pt 1: 305-9, 1998 Jan.
Article
de En
| MEDLINE
| ID: mdl-9542101
ABSTRACT
The nucleotide sequence of the 16S rRNA genes of four rare human mycoplasma species, Mycoplasma faucium, M. buccale, M. primatum and M. spermatophilum, were partially sequenced and compared to published rRNA genes of mycoplasmas to determine their position in the Mollicutes phylogenetic tree. Nucleotide sequence motif and overall similarities allowed positioning of these mycoplasmas in the hominis phylogenetic group, as defined by Weisburg et al. [Weisburg, W. G., Tully, J. G., Rose, D. L. & 9 other authors (1989). J Bacteriol 171, 6455-6467]. Furthermore, these mycoplasmas could be clustered into two different subdivisions of the hominis group (i) M. faucium and M. buccale were found to be included in the M. fermentans subdivision, and (ii) M. primatum and M. spermatophilum were included in the M. hominis one. Variable regions of the 16S rRNA genes were used to determine specific PCR primers to detect and identify M. faucium.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
ARN ribosomique 16S
/
Mycoplasma
/
Infections à Mycoplasma
Type d'étude:
Prognostic_studies
Limites:
Humans
Langue:
En
Journal:
Int J Syst Bacteriol
Année:
1998
Type de document:
Article
Pays d'affiliation:
France