Association between interleukin-4-producing T lymphocyte frequencies and reduced risk of graft-versus-host disease.
Transplantation
; 65(7): 979-88, 1998 Apr 15.
Article
de En
| MEDLINE
| ID: mdl-9565104
ABSTRACT
BACKGROUND:
We have previously developed and used limiting dilution analysis to measure frequencies of alloreactive cytotoxic T cell precursors (CTLp) and interleukin (IL)-2-producing T helper cells (IL-2/HTLp) to assess the risk of graft-versus-host disease in bone marrow transplantation (BMT). However, no test has been available to measure precursor frequencies of the important IL-4-secreting subset.METHODS:
We have now established a limiting dilution analysis to measure the frequency of IL-4-producing T helper cells (IL-4/HTLp) using the IL-4-responsive indicator cell line CT.h4S and have applied this assay to measure alloreactive IL-4/HTLp frequencies in BMT donor-recipient pairs. These frequencies were then analyzed in the context of clinical data to assess the relationship between the number of donor anti-recipient IL-4-secreting T cells and disease outcome.RESULTS:
Frequencies of IL-4/HTLp have been studied in HLA-identical siblings, HLA-"matched" unrelated, and HLA-mismatched combinations and found to range from approximately 1/500,000 in HLA-identical sibling pairs to -1/2,000 in HLA-DR-mismatched pairs. These frequencies were independent of those for IL-2/HTLp and showed a negative correlation with those for CTLp. Clinical follow-up of 30 patients showed that high IL-4/HTLp frequencies are associated with a reduced risk of severe graft-versus-host disease. High IL-4/HTLp frequencies may also indicate an increased risk of leukemia relapse.CONCLUSIONS:
Our data suggest that measurement of IL-4/HTLp frequencies provides information distinct from that obtained with CTLp and IL-2/HTLp. This new assay provides a valuable additional method for optimizing donor selection in unrelated BMT.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Interleukine-4
/
Lymphocytes T auxiliaires
/
Maladie du greffon contre l'hôte
Type d'étude:
Etiology_studies
/
Risk_factors_studies
Limites:
Animals
/
Humans
Langue:
En
Journal:
Transplantation
Année:
1998
Type de document:
Article
Pays d'affiliation:
Royaume-Uni