PACSIN, a brain protein that is upregulated upon differentiation into neuronal cells.
Eur J Biochem
; 256(1): 201-11, 1998 Aug 15.
Article
de En
| MEDLINE
| ID: mdl-9746365
ABSTRACT
To identify genes that are differentially expressed during self-repair processes in mouse brain, we screened a subtracted cDNA library enriched for brain-specific clones. One of these clones, H74, detected a 4.4-kb mRNA predominantly expressed in brain and dorsal root ganglia neurons. Expression increased continuously during the lifespan and the state of differentiation, but decreased after entorhinal-cortex lesion. A full-length cDNA clone was isolated from a cerebellum cDNA library and characterized. Sequence analysis and database search revealed high sequence similarity to FAP52, a protein expressed in focal-adhesion contacts, and uncharacterized Echinococcus and Caenorhabditis elegans gene products. Furthermore, peptide sequences derived from human cDNA fragments showed up to 65% sequence identity at the amino acid level. The presence of a C-terminal src homology 3 (SH3) domain and its phosphorylation by casein kinase 2 (CK2) and protein kinase C (PKC) imply a role in signaling. Here we demonstrate that the gene encodes a phosphoprotein, referred to as PACSIN, with a restricted spatial and temporal expression pattern.
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Phosphoprotéines
/
Neuropeptides
/
Cortex entorhinal
/
Régénération nerveuse
/
Neurones
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Eur J Biochem
Année:
1998
Type de document:
Article
Pays d'affiliation:
Allemagne