A vector for the expression of recombinant monoclonal Fab fragments in bacteria.
J Immunol Methods
; 217(1-2): 195-9, 1998 Aug 01.
Article
de En
| MEDLINE
| ID: mdl-9776589
ABSTRACT
The availability of genes coding for monoclonal Fab fragments of a desired specificity permits their expression in bacteria and provides a simple method for the generation of good quality reagents. In this paper we describe a new phagemid vector for the production of recombinant Fabs from genes obtained from phage display combinatorial libraries. The phagemid features an antibiotic resistance cassette which, once inserted between the heavy chain fragment and the light chain genes, avoids unwanted recombination and preserves useful restriction sites not affecting the Fab production rate.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Fragments Fab d'immunoglobuline
/
Régulation de l'expression des gènes
/
Vecteurs génétiques
/
Anticorps monoclonaux
Limites:
Humans
Langue:
En
Journal:
J Immunol Methods
Année:
1998
Type de document:
Article
Pays d'affiliation:
Italie