Detection of an anhydride intermediate in the carboxypeptidase A catalyzed hydrolysis of a peptide substrate by solid state NMR spectroscopy and its mechanistic implication.
Bioorg Med Chem Lett
; 8(23): 3379-84, 1998 Dec 01.
Article
de En
| MEDLINE
| ID: mdl-9873738
ABSTRACT
We have detected an anhydride intermediate in the CPA catalyzed proteolytic reaction of Gly-Tyr. It appears that since the zinc-bound water molecule which is believed to attack the scissile amide carbonyl carbon in the hydrolysis reaction is excluded by the N-terminal amino group of Gly-Tyr, the carboxylate of Glu-270 becomes to attack the amide bond to generate the anhydride intermediate.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Carboxypeptidases
/
Dipeptides
/
Anhydrides
Type d'étude:
Diagnostic_studies
Langue:
En
Journal:
Bioorg Med Chem Lett
Sujet du journal:
BIOQUIMICA
/
QUIMICA
Année:
1998
Type de document:
Article