Culturing Schwann cells by explants and modified enzyme digestion technique / 中华神经医学杂志
Chinese Journal of Neuromedicine
; (12): 461-463, 2011.
Article
de Zh
| WPRIM
| ID: wpr-1033263
Bibliothèque responsable:
WPRO
ABSTRACT
Objective To introduce an efficient method for culturing and purifying Schwann cells (SCs) in vitro. Methods Sciatic nerves were harvested from neonatal SD rats and the epineuria were removed. Single enzyme digestion combined with explans and double enzyme digestion were employed, respectively, to digest the nerve tissues following trituration. The proliferation of SCs and degree of purification were evaluated by viable count method and the combination of S-100 labeled monoclonal antibody and SCs. Results Proximally 3.5 × 106 cells were harvested with 96% survival rate and a purity of Schwann cells over 94% by single enzyme digestion combined with explans, however only 3.0×106 cells were gained with the purity being 90% and survival rate being 92% by double enzyme digestion. Conclusion This method yields large amount of viable Schwann cells with high purity and survival rate.
Texte intégral:
1
Base de données:
WPRIM
Langue:
Zh
Journal:
Chinese Journal of Neuromedicine
Année:
2011
Type de document:
Article