Inhibitory effect of arctigenin on lymphocyte activation stimulated with PMA/ionomycin / 药学学报
Acta Pharmaceutica Sinica
; (12): 482-489, 2014.
Article
de En
| WPRIM
| ID: wpr-245058
Bibliothèque responsable:
WPRO
ABSTRACT
This study investigated the effect of arctigenin (Arc) on the cell activation, cytokines expression, proliferation, and cell-cycle distribution of mouse T lymphocytes. Mouse lymphocytes were prepared from lymph node and treated with Phorbol-12-myristate-13-acetate (PMA)/Ionimycin (Ion) and/or Arc. CD69, CD25, cytokines, proliferation and cell cycle were assayed by flow cytometry. The results showed that, at concentrations of less than 1.00 micromol x L(-1), Arc expressed non-obvious cell damage to cultured lymphocytes, however, it could significantly down-regulate the expression of CD69 and CD25, as well as TNF-alpha, IFN-gamma, IL-2, IL-4, IL-6 and IL-10 on PMA/Ion stimulated lymphocytes. At the same time, Arc could also inhibit the proliferation of PMA/Ion-activated lymphocytes and exhibited lymphocyte G 0/G1 phase cycle arrest. These results suggest that Arc possesses significant anti-inflammatory effects that may be mediated through the regulation of cell activation, cytokines expression and cell proliferation.
Texte intégral:
1
Base de données:
WPRIM
Sujet principal:
Pharmacologie
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Plantes médicinales
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12-Myristate-13-acétate de phorbol
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Activation des lymphocytes
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Lymphocytes T
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Antigènes de différenciation des lymphocytes T
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Antigènes CD
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Ionomycine
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Chimie
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Cytokines
Limites:
Animals
Langue:
En
Journal:
Acta Pharmaceutica Sinica
Année:
2014
Type de document:
Article