Proteomic study of the effect of recombinant hFGF-10 adenovirus on HaCat cells / 药学学报
Acta Pharmaceutica Sinica
; (12): 55-59, 2010.
Article
de Zh
| WPRIM
| ID: wpr-250620
Bibliothèque responsable:
WPRO
ABSTRACT
This study is to investigate the effect of recombinant hFGF-10 adenovirus on the proteome of HaCat cells, and to speculate further the possible mechanism of the effect of hFGF-10 on HaCat cells via differentially expressed proteins identified. Two-dimensional gel electrophoresis (2-DE) combined with tandem time-of-flight mass spectrometry was applied to identify the differentially expressed protein spots on the 2-DE maps of the whole-cell proteins from Ad infected and rAd-hFGF-10 infected HaCat cells. The mRNA and protein levels of the differentially expressed proteins were confirmed with semi-quantitative RT-PCR and Western blotting. The results showed that the 2-DE maps with high resolution were obtained, and four selected differentially expressed proteins involved in cell apoptosis, cytoskeleton regulation and protein degradation were identified with MALDI-TOF/TOF. The mRNA and protein levels of one of the differentially expressed proteins, VDAC2, were up-regulated in HaCat cells infected with the recombinant hFGF-10 adenovirus. The differentially expressed protein, VDAC2, may be related to the bioactivities of hFGF-10.
Texte intégral:
1
Base de données:
WPRIM
Sujet principal:
Protéines recombinantes
/
ARN messager
/
Transfection
/
Électrophorèse bidimensionnelle sur gel
/
Kératinocytes
/
Régulation positive
/
Lignée cellulaire
/
Adenoviridae
/
Spectrométrie de masse MALDI
/
Biologie cellulaire
Type d'étude:
Prognostic_studies
Limites:
Humans
Langue:
Zh
Journal:
Acta Pharmaceutica Sinica
Année:
2010
Type de document:
Article