Pharmacokinetic study on spinosin in rat plasma after oral administration of suanzaoren extract at a single dose / 药学学报
Acta Pharmaceutica Sinica
; (12): 448-450, 2003.
Article
de En
| WPRIM
| ID: wpr-251064
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To study the pharmacokinetics of spinosin in rat plasma after oral administration of Suanzaoren extract using sulfamethoxazole (SMZ) as internal standard by RP-HPLC method.</p><p><b>METHODS</b>Plasma samples were deproteined with acetonitrile, followed by evaporation of the acetonitrile to dryness. The residual was then resolved in mobile phase and HPLC separation was achieved on a Hypersil C18 (5 microns, 200 mm x 4.6 mm ID) column at 35 degrees C. The mobile phase consisted of acetonitrile-water-acetic acid (15851) at a flow rate of 0.7 mL.min-1. The UV detection wavelength was set at 334 nm.</p><p><b>RESULTS</b>The calibration curve was shown to be linear over the range from 18.1 to 903.5 micrograms.L-1 (r2 > or = 0.995). Mean recovery was 94.5%. Within-day and between-day precisions RSD were less than 9.0%. The limit of quantitation was 18.1 micrograms.L-1. The plasma spinosin was stable at -20 degrees C.</p><p><b>CONCLUSION</b>The simple, sensitive and accurate HPLC method developed has been applied to determine the pharmacokinetics of spinosin in rat plasma after having taken Suanzaoren extract at a single dose.</p>
Texte intégral:
1
Base de données:
WPRIM
Sujet principal:
Plantes médicinales
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Graines
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Flavonoïdes
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Sang
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Médicaments issus de plantes chinoises
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Pharmacocinétique
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Chimie
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Administration par voie orale
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Chromatographie en phase liquide à haute performance
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Rat Wistar
Limites:
Animals
Langue:
En
Journal:
Acta Pharmaceutica Sinica
Année:
2003
Type de document:
Article