Simultaneous determination of multicomponent in Xuefuzhuyu granule with different UV-wavelength by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish the method for the quality control of Xuefuzhuyu granule.</p><p><b>METHOD</b>The content of Semen Armeniacae Amarum PE, Hydroxysafflor yellow A, paeoniflorin and ferulic acid in Xuefuzhuyu granule were determined by HPLC under multi-wavelength of 210, 403, 230, 316 nm.</p><p><b>RESULT</b>The calibration curve of Semen Armeniacae Amarum PE. hydroxysafflor yellow A, paeoniflorin and ferulic acid were linear within 0.1154-0.9232 (r = 0.9998), 0.0219-0.1754 (r = 0.9994), 0.3952-3.1616 (r = 0.9995), and 0.0426-0.3408 microg (r = 0.9998), respectively. The average recoveries were 101.78% (RSD was 1.7%), 99.60% (RSD was 2.9%), 98.90% (RSD was 2.0%), and 100.31% (RSD was 1.8%), respectively.</p><p><b>CONCLUSION</b>The method of quantification is accurate, rapid and reliable for the quality control of Xuefuzhuyu granule.</p>