Cloning and bioinformatic analysis of FatB genes in Lonicera japonica Thunb and its substitutes / 药学学报
Acta Pharmaceutica Sinica
; (12): 1394-1398, 2012.
Article
de Zh
| WPRIM
| ID: wpr-274648
Bibliothèque responsable:
WPRO
ABSTRACT
A FatB unigene was obtained from the transcriptome dataset of Lonicera japonica Thunb. Full-length FatB cDNA was cloned from buds of Lonicera japonica Thunb., Lonicera japonica Thunb. var. chinensis (Wats.) Bak., Lonicera hypoglauca Miq. and Lonicera dasystyla Rehd. using RT-PCR technology, and named as LJFatB, LHFatB, LJCFatB and LDFatB. The results of bioinformatic analysis showed that LJFatB, LJCFatB, LHFatB and LDFatB and Arabidopsis thaliana AtFatB had a closely relationship. Nucleotide sequences and protein secondary structure of LJFatB, LJCFatB, LHFatB and LDFatB are different and their proteins had conserved FatB substrate binding sites and catalytic activity sites. Transcriptive level of LJFatB, LJCFatB, LHFatB and LDFatB in bud was not significantly different. Therefore, LJFatB, LJCFatB, LHFatB and LDFatB could have the same biological function as AtFatB.
Texte intégral:
1
Base de données:
WPRIM
Sujet principal:
Phylogenèse
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Protéines végétales
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Thiolester hydrolases
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Chimie
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Cadres ouverts de lecture
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Séquence d'acides aminés
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Classification
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Clonage moléculaire
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Structure secondaire des protéines
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ADN complémentaire
Langue:
Zh
Journal:
Acta Pharmaceutica Sinica
Année:
2012
Type de document:
Article