Expression of Brain-derived Neurotrophic Factor and Tyrosine Kinase B in Cerebellum of Poststroke Depression Rat Model / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 2926-2931, 2015.
Article
de En
| WPRIM
| ID: wpr-275593
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>The pathophysiology of poststroke depression (PSD) remains elusive because of its proposed multifactorial nature. Accumulating evidence suggests that brain-derived neurotrophic factor (BDNF) plays a key role in the pathophysiology of depression and PSD. And the cerebellar dysfunction may be important in the etiology of depression; it is not clear whether it also has a major effect on the risk of PSD. This study aimed to explore the expression of BDNF and high-affinity receptors tyrosine kinase B (TrkB) in the cerebellum of rats with PSD.</p><p><b>METHODS</b>The rat models with focal cerebral ischemic were made using a thread embolization method. PSD rat models were established with comprehensive separate breeding and unpredicted chronic mild stress (UCMS) on this basis. A normal control group, depression group, and a stroke group were used to compare with the PSD group. Thirteen rats were used in each group. Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) for detecting the expression of BDNF and TrkB protein and mRNA in the cerebellum were used at the 29 th day following the UCMS.</p><p><b>RESULTS</b>Compared with the normal control group and the stroke group, the number of BDNF immunoreactive (IR) positive neurons was less in the PSD group (P < 0.05). Furthermore, the number of TrkB IR positive cells was significantly less in the PSD group than that in the normal control group (P < 0.05). The gene expression of BDNF and TrkB in the cerebellum of PSD rats also decreased compared to the normal control group (P < 0.05).</p><p><b>CONCLUSIONS</b>These findings suggested a possible association between expression of BDNF and TrkB in the cerebellum and the pathogenesis of PSD.</p>
Texte intégral:
1
Base de données:
WPRIM
Sujet principal:
Protein-tyrosine kinases
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Cervelet
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Rat Sprague-Dawley
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Facteur neurotrophique dérivé du cerveau
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Accident vasculaire cérébral
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Dépression
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Métabolisme
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Chinese Medical Journal
Année:
2015
Type de document:
Article