Construction of a novel gene therapy lentiviral vector for drug resistant selection and detection in vivo / 生物工程学报
Chinese Journal of Biotechnology
; (12): 256-261, 2008.
Article
de Zh
| WPRIM
| ID: wpr-276130
Bibliothèque responsable:
WPRO
ABSTRACT
Lentiviral vectors were powerful gene delivery tools for gene therapy. We developed a new lentiviral vector pBobi-MIL that constitutively expressed O6-methylguanine-DNAmethyltransferase (MGMT) and Luciferase, linked by the internal ribosomal entry site (IRES), to realize drug tolerance and real time monitoring in vivo. All results from RT-PCR, drug treating clones forming, immunofluorometric assay and chemiluminescence detection showed that cells infected by recombinant lentivirus L-MIL simultaneously expressed these two genes. This lays the foundation for the further research in gene therapy and can also help identify lentivirus titer.
Texte intégral:
1
Base de données:
WPRIM
Sujet principal:
Résistance aux substances
/
DNA modification methylases
/
Thérapie génétique
/
Lentivirus
/
Protéines suppresseurs de tumeurs
/
Enzymes de réparation de l'ADN
/
Vecteurs génétiques
/
Génétique
/
Luciferases
/
Métabolisme
Type d'étude:
Diagnostic_studies
Limites:
Humans
Langue:
Zh
Journal:
Chinese Journal of Biotechnology
Année:
2008
Type de document:
Article