Extract of Zuojin Pill (characters: see text) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 837-845, 2015.
Article
de En
| WPRIM
| ID: wpr-287180
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of water extract of Zuojin Pill ([characters: see text], ZJP) on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism.</p><p><b>METHODS</b>Effects of ZJP on SGC-7901 cells growth were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, cell apoptosis and cell cycle were determined by flow cytometry, and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell, mitochondrial membrane potentials, expression of apoptosis-related genes, such as B cell lymphoma/lewkmia-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cleaved caspase-3 and caspase-9.</p><p><b>RESULTS</b>ZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells, leading to attenuation of mitochondrial membrane potential and DNA fragmentation.</p><p><b>CONCLUSIONS</b>ZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent.</p>
Mots clés
Texte intégral:
1
Base de données:
WPRIM
Sujet principal:
Pharmacologie
/
Médicaments issus de plantes chinoises
/
Survie cellulaire
/
Technique de Western
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Espèces réactives de l'oxygène
/
Apoptose
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Colorimétrie
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Test des comètes
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Lignée cellulaire tumorale
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Membranes mitochondriales
Limites:
Humans
Langue:
En
Journal:
Chinese journal of integrative medicine
Année:
2015
Type de document:
Article