Expression of somatostatin receptor reporter gene and its correlation with targeted imaging in vivo for detection of pancreas carcinoma / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 663-666, 2005.
Article
de Zh
| WPRIM
| ID: wpr-308470
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the value of (99 m)Tc-sandostatin scintigraphy in targeted diagnosis of pancreas carcinoma and the correlation with expression of somatostatin receptor (SSTR) reporter gene in tumor tissue.</p><p><b>METHODS</b>Nude mice bearing human pancreas carcinoma xenograft were established in advance. (99 m)Tc-sandostatin imaging was performed in 18 nude mice and tumor to normal tissue ratio (T/NT) was calculated. mRNA expression of SSTR1, SSTR2 and SSTR5 in tumor tissue was detected by RT-PCR.</p><p><b>RESULTS</b>Out of 18 nude mice, 13 mice showed intense uptake of (99 m)Tc-sandostatin. Six hours after (99 m)Tc-sandostatin administration, the T/NT ratio was 2.53 +/- 0.84. Five mice showed negative findings, and the T/NT ratio was 1.04 +/- 0.06. Positive correlations were obtained between the T/NT ratio and the expression of SSTR1 and SSTR2 mRNA, especially SSTR2 (r = 0.807, P < 0.01).</p><p><b>CONCLUSION</b>High expression of SSTR1, SSTR2 and SSTR5 mRNA were found in human pancreas tumor xenograft in nude mice, especially SSTR2. There is a significant correlation between the tumor uptake of (99 m)Tc-sandostatin and SSTR2 mRNA level. This approach may allow SSTR-targeted diagnosis and therapy of human pancreas cancer.</p>
Texte intégral:
1
Base de données:
WPRIM
Sujet principal:
Tumeurs du pancréas
/
ARN messager
/
Imagerie diagnostique
/
Cellules cancéreuses en culture
/
Octréotide
/
Scintigraphie
/
Composés organiques du technétium
/
Récepteur somatostatine
/
Gènes rapporteurs
/
Génétique
Type d'étude:
Diagnostic_studies
Limites:
Animals
/
Humans
/
Male
Langue:
Zh
Journal:
Chinese Journal of Oncology
Année:
2005
Type de document:
Article