Synthesis and identification of rutin complete antigen and analysis its immunogenicity / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 397-401, 2013.
Article
de Zh
| WPRIM
| ID: wpr-346809
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Synthesis and identification of complete antigen of rutin, the traditional Chinese medicine active ingredient, and develop rapid detection of rutin using enzyme-linked immunoassay method (ELISA). Immunogenicity of the complete antigen was also studied.</p><p><b>METHOD</b>Prepare the complete antigen by sodium periodate solution and identified by UV scanning and SDS-PAGE test. Male New Zealand white rabbits were immunized by the antigen to obtain the antiserum.</p><p><b>RESULT</b>The results of UV analysis showed that the coupling ratio of complete antigen is 13: 1. SDS-PAGE display of the artificial antigen was delayed compared with bovine serum protein. The titer of rutin antibody is 1:4 000. The sensitivity of IC50 was 5.37 mg x L(-1), the lowest detection limit was 1 mg x L(-1), the average recovery was 102%, the intra and interspecific RSD were less than 10%, cross-reactivity rate of antibodies and other analogs were less than 1%.</p><p><b>CONCLUSION</b>Rutin complete antigen was synthesized successfully, and the rapid detection of rutin by ELISA method was successfully established.</p>
Texte intégral:
1
Base de données:
WPRIM
Sujet principal:
Rutoside
/
Solutions
/
Sérumalbumine bovine
/
Test ELISA
/
Chimie
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Immunisation
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Acide orthoperiodique
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Réactions croisées
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Électrophorèse sur gel de polyacrylamide
/
Allergie et immunologie
Type d'étude:
Diagnostic_studies
Limites:
Animals
Langue:
Zh
Journal:
China Journal of Chinese Materia Medica
Année:
2013
Type de document:
Article