Effect of neonatal exposure to environmental pollutants on the DNA methylation of rat testis / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 649-654, 2009.
Article
de Zh
| WPRIM
| ID: wpr-352821
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of neonatal exposure of DNA methylation inhibitor, Cadmium and PCB153 on DNA methylation, apoptosis and spermatogenesis in SD rats.</p><p><b>METHODS</b>Neonatal SD rats were randomly divided into 10 groups and received oral administrations of PCB153 (0.025, 0. 250, 2.500 mg/kg), or Cadmium (1, 2, 4 mg/kg), or positive control 5-Aza-CdR (0.025, 0.250 mg/kg), or vehicle control for five days from PND3. Half of the rats were killed 24 h after the last administration. The remains were fed until 12 weeks. Sperm numbers, apoptosis and DNA methylation levels in testis were investigated.</p><p><b>RESULTS</b>The daily sperm production was significantly decreased in each neonatal exposed group (P < 0.05). Neonatal rats exposed to 5-Aza-CdR and Cadmium reduced the global DNA methylation level, increased apoptosis, while PCB153 exposure did not significantly change DNA methylation and apoptosis.</p><p><b>CONCLUSION</b>Neonatal rats exposed to chemicals could reduce spermatogenesis via multiple pathways. Lower DNA methylation and increased neonatal apoptosis were suggested as one of the causes.</p>
Texte intégral:
1
Base de données:
WPRIM
Sujet principal:
Anatomopathologie
/
Spermatogenèse
/
Testicule
/
Cadmium
/
Polychlorobiphényles
/
Rat Sprague-Dawley
/
Apoptose
/
Méthylation de l'ADN
/
Toxicité
/
Animaux nouveau-nés
Limites:
Animals
Langue:
Zh
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Année:
2009
Type de document:
Article