Characterization and Diagnostic Use of a Monoclonal Antibody for VP28 Envelope Protein of White Spot Syndrome Virus / 中国病毒学·英文版
Virologica Sinica
; (6): 260-266, 2011.
Article
de Zh
| WPRIM
| ID: wpr-423780
Bibliothèque responsable:
WPRO
ABSTRACT
The gene encoding the VP28 envelope protein of White spot syndrome virus (WSSV)was cloned into expression vector pET-30a and transformed into the Escherichia coli strain BL21.After induction,the recombinant VP28 (rVP28) protein was purified and then used to immunize Balb/c mice for monoclonal antibody (MAb) production.It was observed by immuno-electron microscopy the MAbs specific to rVP28 could recognize native VP28 target epitopes of WSSV and dot-blot analysis was used to detect natural WSSV infection.Competitive PCR showed that the viral level was approximately 104 copies/mg tissue in the dilution of gill homogenate of WSSV-infected crayfish at the detection limit of dot-blot assay.Our results suggest that dot-blot analysis with anti-rVP28 MAb could rapidly and sensitively detect WSSV at the early stages of WSSV infection.
Texte intégral:
1
Base de données:
WPRIM
Type d'étude:
Diagnostic_studies
Langue:
Zh
Journal:
Virologica Sinica
Année:
2011
Type de document:
Article