Contents Determination of Chlorogenic Acid and Vitexin in Lophatherum gracile by HPLC / 中国药房
China Pharmacy
; (12): 833-834,835, 2016.
Article
de Zh
| WPRIM
| ID: wpr-605264
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE:To establish a method for the simultaneous determination of chlorogenic acid and vitexin in Lophather-um gracile. METHODS:HPLC was performed on the column was Waters Atlantis C18 with mobile phases of acetonitrile- water (gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 280 nm,column temperature was 35 ℃,and the injec-tion volume was 10 μl. RESULTS:The linear range was 0.041 0-1.228 8 μg for chlorogenic acid(r=0.999 8)and 0.264 0-7.920 0μg for vitexin(r=0.999 9);RSDs of precision, stability and reproducibility tests were lower than 2%;recoveries were 97.6%-102.3%(RSD=1.85%,n=9) and 97.1%-101.3%(RSD=1.19%,n=9),respectively. CONCLUSIONS:The method is simple,stable and reproducible,and can be used for the simultaneous determination of chlorogenic acid and vitexin in L. gracile.
Texte intégral:
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Base de données:
WPRIM
Langue:
Zh
Journal:
China Pharmacy
Année:
2016
Type de document:
Article